CONTROL TRIALS
(for
more information:
Espadaler, X. Bernal, V. i Rojo, M. 2006b.)
- As in
other Lasius species, there is a short period in spring with
a reduced level of predatory activity. Nontheless, Lasius
brunneus is better characterized as an aphid-tending species.
- The number of workers within the colony is unknown but
likely to be very high. Probably > 10,000 in a mature colony.
- The location, both of the ants as well as the aphids, is
under the bark, especially at the non-exploited zone of the
cork. This renders extremely difficult, if not useless, any
insecticide spray treatment.
- Biological control is not considered applicable. No
natural enemies are currently known for Lasius brunneus.
The obvious option is, therefore, the use of delayed-effect
baits This is currently the control treatment of choice for
other pest ants, like the Argentine ant Linepithema humile (Mayr)
whose societies are constituted of huge numbers of
individuals and the feeding of whose workers is mainly
liquid-based.
Preliminary field tests have been carried out. Baits with
molasses as excipient were highly attractive. Consumption of
baits was independent of the physical localization of baits
within the tree (base of trunk, mid-height of the trunk, or
where the trunk branches out).
The application of baits was carried out by means of darkened Eppendorf containers, additionally covered with
opaque paper, to reduce the quantity of light inside the
container. This was done to counter the light-avoiding
escape reaction, characteristic of these ants. In the summer
of 2004 we tested the effectiveness of baits based on
clorpirifos (0.075%), which had been shown to be effective
in laboratory. During 2005, new baits were used: a) an
artificial diet with borax (6%) as active ingredient, b)
commercial Blattanex® baits, and c) a new experimental bait
without molasses and with clorpirifos (2%; Mylva CORP.).
Finally, during 2006, different carriers were used using the
boric acid as active matter (Mylva CORP.) at two different
concentrations (3 or 5%). The higher boric acid
concentrations gave only very partially satisfactory results.
Considering the location of the ants underneath the cork,
we have to resort to indirect indicators in order to measure
the effect of the treatments. The replacement of sawdust in
the vertical slits by the workers of Lasius brunneus to protect
themselves from the light, constitutes a direct and easy to
measure variable. The process is as follows: 1) for every
tree, the cumulative length (cm) of sawdust in the vertical
slits and cracks is measured; 2) sawdust is then eliminated
with a brush; 3) the bait treatment is administered; 4) the
replacement of sawdust is checked every two weeks until
hibernation begins; 5) a last measure is taken the following
year in summer. The rationale assumption is that the lesser
is the replacement of sawdust, the better is the state of
the colony. For control trees, bait administration (step 3)
is not applied.
A. Recovery of
sawdust in the treated and control cork-oaks. No significant
differences between the three treatments were detected (2004). A
last measurement made in 2005 is added to discard a possible
delayed effect in time.
B. Absence of
relationship
between the amount (g) of consumed bait and the recovery of
sawdust (proportion) (2004).
C. Recovery of
sawdust in the treated and control cork-oaks. No significant
differences between the treatments were detected (2005).
D. Absence of
relationship
between the amount (g) of consumed bait and the recovery of
sawdust (proportion) (2005). In the Blattanex baits the
consumption was not evaluated.
E. Recovery of
sawdust in the treated and control cork-oaks. No significant
differences between the treatments were detected (2006).
F. Significant relationship between the quantity (g) of consumed
active ingredient and sawdust recovery (in %) (2006). |